Team Barnyard is the name of a team of crime-fighting animals led by Otis. These friends often have adventures together and fight villains from all over the county.
- Otis- A carefree male cow who loves to party. He is the leader of the group.
- Pip- A wisecracking mouse, who is also Otis' best friend. He is the co-leader.
- Pig- A filthy and gluttonous pig. He is the only member to be named after his own species. He is the brawn of the group.
- Freddy- A frisky and crazy ferret, who often gets tempted to eat Peck or Pip. He is the clown of group.
- Peck- A rooster who is also Freddy's best friend. He is the brains of the group.
- Duke- The farmer's sheepdog, who wants Otis' position as leader. He is the mascot of the group.
- Miles- A wise donkey, who serves as a father figure to Otis and his friends. He is the group's voice of reason.
- Abby- A female cow, who is Otis' love interest. She is the ditz of the group.
- Bessy- Another female cow, who Pip has a crush on and is Abby's best friend. She is the secondary brawn of the group.
- The Jersey Cows- Three troublemaking steers who love to play pranks. They are the jocks of the gang.