Daisy used to live on a farm, had a husband, and was pregnant with her first calf. She enjoyed her life with her family, but then a flash flood rolled into the farm. She and Bessy found refuge in the meadow, but when the storm was over, everybody was killed. Later, the Farmer took her and Bessy and they met the other animals.
At the start of the film, she was rather shy due to being new to the Barnyard, but Otis made her and Bessy feel welcome.
She has a loving relationship with Otis.
Daisy is a cream-brown colored cow with a pink snout and udders.
- It is never revealed what happened to Daisy and Lil' Ben between the events of the film and the series or when Abby joined.